Possible Rotation Projects:
Mechanisms of reovirus neurotropism
Formation of reovirus replication organelles
Non-cytolytic viral egress from cells
Training Technologies Used:
A variety of genetic, biochemical, and cell biological techniques are used to study viral infection and disease.
- B.S., Biology, Cornell University, 1978
- MD, Medicine, Columbia University College of Physicians and Surgeons, 1982
Barton, E. S., Forrest, J. C., Connolly, J. L., Chappell, J. D., Liu, Y., Schnell, F. J., Nusrat, A., Parkos, C. A., and T. S. Dermody. Junction adhesion molecule is a receptor for reovirus. Cell 104, 441-451 (2001). PMID: 11239401
Ebert, D. H., Deussing, J., Peters, C., and T. S. Dermody. Cathepsin L and cathepsin B mediate reovirus disassembly in murine fibroblast cells. J. Biol. Chem. 277, 24609-24617 (2002). PMID: 11986312
Kobayashi, T., Antar, A. A. R., Boehme, K. W., Danthi, P., Eby, E. A., Guglielmi, K. M., Holm, G. H., Johnson, E. M., Maginnis, M. S., Naik, S., Skelton, W. B., Wetzel, J. D., Wilson, G. J., Chappell, J. D., and T. S. Dermody. A plasmid-based reverse genetics system for animal double-stranded RNA viruses. Cell Host Microbe 1, 147-157 (2007). PMCID: PMC2034303
Antar, A. A. R., Konopka, J. L., Campbell, J. A., Henry, R. A., Perdigoto, A. L., Carter, B. D., Pozzi, A., Abel, T. W., and T. S. Dermody. Junctional adhesion molecule-A is required for hematogenous dissemination of reovirus. Cell Host Microbe. 5, 59-71 (2009). PMCID: PMC2642927
Konopka-Anstadt, J. L., Mainou, B. A., Sutherland, D. M, Sekine, Y., Strittmatter, S. M., and T. S. Dermody. The Nogo receptor NgR1 mediates infection by mammalian reovirus. Cell Host Microbe 15, 681-691 (2014). PMCID: PMC4100558
Bouziat, R., Hinterleitner, R., Brown, J. J., Stencel-Baerenwald, J. E., Ikizler, M., Mayassi, T., Meisel, M., Kim, S. M., Discepolo, V., Pruijssers, A. J., Ernest, J. D., Iskarpatyoti, J. A., Costes, L. M. M., Lawrence, I., Palanski, B. A., Varma, M., Zurenski, M. A., Khomandiak, S., McAllister, N., Aravamudhan, P., Boehme, K. W., Hu, F., Samsom, J. N., Reinecker, H.-C., Kupfer, S. S., Guandalini, S., Semrad, C. E., Abadie, V., Khosla, C., Barreiro, L. B., Xavier, R. J., Ng, A., Dermody, T. S.,* and B. Jabri.* Reovirus infection breaks tolerance to dietary antigens and promotes development of celiac disease. Science 356, 44-50 (2017). *Co-corresponding authors. PMCID: PMC5506690
Knowlton, J. J., Fernández, I., Ashbrook, A. W., Gestaut, D. R., Zamora, P. F., Bauer, J. A., Forrest, J. C., Frydman, J., Risco, C., and T. S. Dermody. The TRiC chaperonin controls reovirus replication through outer-capsid folding. Nat. Microbiol. 3, 481-493 (2018). PMCID: PMC5874176
Sutherland, D. M., Streßl, M., Koehler, M., Welsh, O. L., Yu, X., Hu, L., Dos Santos Natividade, R., Knowlton, J. J., Taylor, G. M., Moreno, R. A., Wörz, P., Lonergan, Z. R., Aravamudhan, P., Guzman-Cardozo, C., Kour, S., Pandey, U. B., Alsteens, D., Wang, Z., Prasad, B. V. V., Stehle, T., and T. S. Dermody. NgR1 binding to reovirus reveals an unusual bivalent interaction and a new viral attachment protein. Proc. Natl. Acad. Sci. USA 120, e2219404120 (2023). PMCID: PMC10268256
Shang, P., Dos Santos Natividade, R., Taylor, G. M., Ray, A., Welsh, O. L., Fiske, K. L., Sutherland, D. M., Alsteens, D., and T. S. Dermody. NRP1 is a receptor for mammalian orthoreovirus engaged by distinct capsid subunits. Cell Host Microbe 32, 980-995 (2024). PMCID: PMC11176008
Somoulay, X., Treadaway, G. S., Hedayati, S., Steed, M. E., Adam, N. E., Williams, M. S., Parker, J. S. L., Woolford, J. L., Silva, L. A., Taylor, G. M., and T. S. Dermody. Ataxin-2-like promotes translation of nonpolyadenylated reovirus mRNA. Nat. Commun. 17,842 (2026). PMCID: PMC12827272
The Dermody laboratory studies the molecular pathogenesis of mammalian reovirus infection. Reovirus is an enteric, neurotropic virus that infects many mammalian species, including humans, but disease is restricted to the very young. Research in the Dermody laboratory encompasses several interrelated themes to better understand viral and cellular determinants of disease. These include viral attachment and entry into cells, genome replication and packaging, egress of viral progeny from infected cells, and functions of viral receptors in disease pathology. We also have established a link between reovirus and blockade of immunologic tolerance to new food antigens.
Projects in our laboratory are available to (i) identify and characterize reovirus receptors and entry strategies, (ii) discover mechanisms of reovirus replication factory formation and function, and (iii) elucidate pathways of reovirus dissemination and tropism in the host and interactions with innate and adaptive immune mediators. We employ genetic screens, biochemical assays, cell imaging, and organismal biology to advance our experimental goals. We collaborate extensively with structural biologists, biophysicists, cell biologists, and neuroscientists and enjoy working with interdisciplinary teams.
